Liquid chromatography offers numerous possibilities how to separate samples of interest. By changing the composition of mobile phase and/or character of stationary phase the separation efficiency and selectivity can be completely change. Read more about main liquid chromatography modes. Size-exclusion chromatography (SEC) Size-exclusion chromatography (also known as gel permeation chromatography, GPC) is separation technique, where [...]
In liquid chromatography liquid mobile phase flows through the column with stationary phase. The main principle of separation remains the same. Compounds have different affinity to the stationary phase and are separated while flowing through the column. The compounds separated with liquid chromatography are disolved in the mobile phase. They have lower difussion coefficients than [...]
Thin layer chromatography is probably the easiest way how to perform chromatographic separation. At least you do not need any instrument. In thin layer chromatography (TLC) the solvent flows through the stationary phase which covers the thin plate. One part of plate is submerged into the mobile phase which travel across the plate using …
The gas chromatography is special type of chromatography, where the mobile phase is gas, such as helium or nitrogen. The stationary phase is usually solid support covered with liquid layer. After the sample injection the mobile phase carries the sample compounds through the column. Usually, the temperature gradient is applied and compounds are then separated [...]
Chromatography is analytical chemistry method which is used (and useful) for the separation of complex mixtures of chemical compounds. The main mechanism of the separation is repeatable distribution of the tested compound in between two different phases. Usually, one phase is solid, fixed in the separation device and the other is moving and flows through [...]
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Chromatographic articles