Archive | August, 2009

Liquid chromatography modes

Liquid chromatography modes

Liquid chromatography offers numerous possibilities how to separate samples of interest. By changing the composition of mobile phase and/or character of stationary phase the separation efficiency and selectivity can be completely change. Read more about main liquid chromatography modes.

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Liquid chromatography

Liquid chromatography

In liquid chromatography liquid mobile phase flows through the column with stationary phase. The main principle of separation remains the same.

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Thin layer chromatography

Thin layer chromatography

Thin layer chromatography is probably the easiest way how to perform chromatographic separation. At least you do not need any instrument. In thin layer chromatography (TLC) the solvent flows through the stationary phase which covers the thin plate. One part of plate is submerged into the mobile phase which travel across the plate using capillary [...]

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Gas chromatography

Gas chromatography

The gas chromatography is special type of chromatography, where the mobile phase is gas, such as helium or nitrogen. The stationary phase is usually solid support covered with liquid layer.

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What is chromatography?

What is chromatography?

Chromatography is analytical chemistry method which is used (and useful) for the separation of complex mixtures of chemical compounds. The main mechanism of the separation is repeatable distribution of the tested compound in between two different phases.

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