Chromatography Forum is a public discussion group where you can post questions, news, or messages of interest to chromatographers everywhere.
I have asked Tom Jupile (founder and moderator) for some factual data about the Chromatography Forum. The forum was started in May 1999 as an adjunct to the LC Resources web site, and was updated to its present format in September 2004. Nowadays, it is probably the busiest chromatography site in the world with about 250 new messages every week. The database has over 57 000 messages (plus another 14 000 or so in the pre-2005 archives), all of which can be searched by keyword.
It is truly an international site: in the past month, it had 15 000 visits from 116 countries, of which almost 2/3 were from outside the US (btw. 69 of them from the Czech Republic;).
Chromatographers: nice community
One thing has to be pointed out. More then half of the Tom’s answer is devoted to the mentality of the chromatographic community. It is nice to hear, that the group of people surrounding the 100 years old separation idea is willing to help and share the ideas, advices and thoughts.
I have been the administrator and moderator since the beginning, but apart from occasional issues with the software (and the internet service!) the amount of effort involved has been minor. Unlike other discussion groups with which I am familiar, the Forum is a remarkably civilized place, with a negligible level of sarcasm, rudeness, and “flames”.
I think this is quite a tribute to the professionalism and generosity of the “chromatography community”: as a group they show a remarkable willingness to share their expertise and knowledge. Over the ten years of the Forum’s existence, I have only ever had to “warn” perhaps a half-dozen people about inappropriate posts, and only ever banned one person. (
Tom Jupille, ChromForum.org moderator
Exactly, chromatographers are nice people!
2 replies on “Chromatography Forum”
Dear chromatographers,
Please, could you let me suggest the HPLC condition of Neomycin sulfate.I use
Column – Hypersil gold C18, 250 X 4.6 mm and ID 5 micrometer
Condition
Eluent – 84% of 0.2 mol Trifluoroacetic acid and 16% of Acetronitrile
Gradient – Isocratic
Flow rate – 0.7mL/min
Detection – Refractive index detector
For that case, I can’t separate two peaks of Neomycin C and B eventhough I changed the different ratios of eluent (90 : 10, 80 : 20, 70 : 30, 60 : 40 and 50 : 50 of 0.2 mol TFA and ACN), they are still coelute. Neomycin has no UV chromophore, so I can’t use UV detector and also our lab don’t have HPLC with ELSD detector. That’s why I use refractive index detector.
Please could someone advice me how should I do?Should I change the mobile phase or should I use derivatization method?Hoping your kind suggestions. Thank you.
Dear Nay,
thank you very much for your question. However, I have to admit I never worked with this type of compounds and I do not have any real experience. On the other hand, have you tried another retention mechanism than RP? For example HILIC on polar stationary phase might be worth trying. Or completely different separation mechanisms like ion-exchange (http://dx.doi.org/10.1016/j.jpba.2006.06.024) or ion-pair chromatography (http://dx.doi.org/10.1016/j.chroma.2004.09.052).
Please let me know if that helped
Have a nice day
Jiri