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Separation of small molecules: surface area vs. gel porosity

Separation of small molecules: surface area vs. gel porosity

Generally, methacrylic acid is used as a charge-bearing agent for generation of electroosmotic flow in capillary electrochromatography. However, methacrylic acid has a significant effect on the morphology of the monolithic stationary phases based on styrene – divinylbenzene system as showed recently by group in Prague. The monolithic material prepared without methacrylic acid in the polymerization [...]

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How to get speed in HPLC

How to get speed in HPLC

Couple of days ago, I mentioned on Chromatographer’s facebook page article by Peter Carr about speed in HPLC published in Analytical Chemistry. There are two parts in the article: (i) critical comparison of different approaches how to reach speed in HPLC and (ii) theoretical background of speed and efficiency optimization in high performance liquid chromatography.

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PF 2011

PF 2011

Last year, I tried to use “chromatographic” New Year’s greeting for first time. It was kind of easy. This year, I would like to continue with this tradition and – additionally to strip presented below – I would like to wish you good health and welfare in the upcoming year. May the bad stuff from [...]

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Communication problem with Chromeleon 6.8

Communication problem with Chromeleon 6.8

Usually, I leave instrument’s computer up and running for long periods of time. No surprise that after several weeks there is significant amount of Windows Updates ready for installation. This week I had to reboot my system (Dionex Ultimate 3000) and during this occasion several new updated were installed. After that I started to have [...]

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One column fits all – Hypercrosslinked monoliths

One column fits all – Hypercrosslinked monoliths

One column fits all was title of my poster presented at HPLC 2010 in Boston that described the preparation and characterization of hypercrosslinked monolithic stationary phases and their application in several chromatographic modes. We summarized poster’s results and submitted them as a paper in Journal of Chromatography A. Hypercrosslinking modification is not a new technique. [...]

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