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Remotely detected NMR and hypercrosslinked monoliths

During the time I spent in Berkeley I had the honor to work on the beginning of the project leading to the portable system for highly sensitive multi-dimensional chemical analysis. This work included hyphenation of NMR with liquid chromatography separation using organic polymer monoliths. I have to admit, it was my first touch of NMR [...]

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Separation of small molecules: surface area vs. gel porosity

Separation of small molecules: surface area vs. gel porosity

Generally, methacrylic acid is used as a charge-bearing agent for generation of electroosmotic flow in capillary electrochromatography. However, methacrylic acid has a significant effect on the morphology of the monolithic stationary phases based on styrene – divinylbenzene system as showed recently by group in Prague. The monolithic material prepared without methacrylic acid in the polymerization [...]

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One column fits all – Hypercrosslinked monoliths

One column fits all – Hypercrosslinked monoliths

One column fits all was title of my poster presented at HPLC 2010 in Boston that described the preparation and characterization of hypercrosslinked monolithic stationary phases and their application in several chromatographic modes. We summarized poster’s results and submitted them as a paper in Journal of Chromatography A. Hypercrosslinking modification is not a new technique. [...]

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My HPLC books

My HPLC books

Today, I would like to describe my favorite chromatographic books: from one I bought even before I (really) knew what chromatography is to one which has chapter with my name on it. Úvod do vysokoúčinné kapalinové kolonové chromatografie I am sorry to all of you who does not understand Czech language. This is my very [...]

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Four directions how to improve monolithic stationary phases

Four directions how to improve monolithic stationary phases

Georges Guiochon pointed out in his excelent reivew about monolithic stationary phases four directions from which we can expect a serious improvement in (monolithic) columns performance. High temperature chromatography High temperature chromatography, which causes a reduction in the viscosity of the mobile phase. So far, monolithic stationary phases have not yet been used at high [...]

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