Chromatography

During the time I spent in Berkeley I had the honor to work on the beginning of the project leading to the portable system for highly sensitive multi-dimensional chemical analysis. This work included hyphenation of NMR with liquid chromatography separation using organic polymer monoliths. I have to admit, it was my first touch of NMR [...]

How do you define chromatography?

by Jiri Urban on May 10, 2010 · 2 comments

How do you define chromatography?

Before the last CASSS Discussion group debate on difference between high temperature and high pressure liquid chromatography started, there was a welcome slide projected on the wall. There was only one sentece (paraphrase): Meet other people who like and understand what you do I highlighted the most important part (for me), because I have always [...]

WebEx. The future of instrumental services?

by Jiri Urban on January 31, 2010 · 0 comments

Online communication

I had to solve connection problem in between the Bruker MS and Agilent LC (Agilent shutdown). On the very end, I found out there was no problem in their mutual communication. However, it shows me the future of the instrumental services. WebEx communication. WebEx is software delivered as a service which you can use it [...]

Merry Christmas and Happy New Year

by Jiri Urban on December 24, 2009 · 0 comments

Happy New Chromatographic Year

Not only from the chromatographic point of view, I wish you in the year 2010: low pressure high efficiency sharp resolution large capacity and satisfactory results … or (in case you dont want to use chromatographic expressions) you can use any of the translations.

Inverse size-exclusion chromatography

by Jiri Urban on October 23, 2009 · 0 comments

Calibration curve in inverse size-exclusion chromatography (Ve/Vc - elution volume of the polymer divided by the volume of the column, log Mr - logarithm of (polystyrene) molar mass)

The inverse application of the size-exclusion chromatography (SEC) concept, inverse size-exclusion chromatography (ISEC) [1], utilizes a set of molecular probes with defined sizes to determine pore dimensions, and is also referred as chromatographic porosimetry [2]. ISEC provides an alternative to mercury porosimetry or nitrogen adsorption for the determination of the pore size dimensions and the surface area of [...]

Thin layer chromatography

by Jiri Urban on August 12, 2009 · 0 comments

Thin layer chromatography

Thin layer chromatography is probably the easiest way how to perform chromatographic separation. At least you do not need any instrument. In thin layer chromatography (TLC) the solvent flows through the stationary phase which covers the thin plate. One part of plate is submerged into the mobile phase which travel across the plate using …

Gas chromatography

by Jiri Urban on August 9, 2009 · 0 comments

Schematic GC instrument

The gas chromatography is special type of chromatography, where the mobile phase is gas, such as helium or nitrogen. The stationary phase is usually solid support covered with liquid layer. After the sample injection the mobile phase carries the sample compounds through the column. Usually, the temperature gradient is applied and compounds are then separated [...]

What is chromatography?

by Jiri Urban on August 7, 2009 · 0 comments

Scheme of chromatography

Chromatography is analytical chemistry method which is used (and useful) for the separation of complex mixtures of chemical compounds. The main mechanism of the separation is repeatable distribution of the tested compound in between two different phases. Usually, one phase is solid, fixed in the separation device and the other is moving and flows through [...]