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How to get speed in HPLC

How to get speed in HPLC

Couple of days ago, I mentioned on Chromatographer’s facebook page article by Peter Carr about speed in HPLC published in Analytical Chemistry. There are two parts in the article: (i) critical comparison of different approaches how to reach speed in HPLC and (ii) theoretical background of speed and efficiency optimization in high performance liquid chromatography.

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Chromatographic coffee cup – do it yourself

Chromatographic coffee cup – do it yourself

Recently, I was browsing amazon.com products related to the chromatography keyword. To my big surprise, the number ten (at least in my results) was a “16 oz. Double Wall Insulated Tumbler with chromatography column alone – Paper Insert”. This is what I call a present for a chromatographer! I waited until our next order (Harry [...]

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High temperature vs. Ultra high pressure liquid chromatography

High temperature vs. Ultra high pressure liquid chromatography

The last CASSS Discussion group focused on the possible advantages and disadvantages of high temperature and/or high pressure in a liquid chromatography. The Discussion group was hold as a debate – two experts against each other. The high temperature approach was defended by Nebojsa M. Djordevic (SANO CRO) and Michael W. Dong (Genetech) advocated the [...]

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Costless improvement of your HPLC instrument

Costless improvement of your HPLC instrument

The recent progress in the column development brought to the market new type of highly efficient columns. These columns can be use either with standard HPLC instruments or with the instruments allowing separations at ultra high pressure (900 MPa). The conventional size of the column (150 x 4.6 mm) has been decreased significantly. The typical [...]

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Inverse size-exclusion chromatography

Inverse size-exclusion chromatography

The inverse application of the size-exclusion chromatography (SEC) concept, inverse size-exclusion chromatography (ISEC) [1], utilizes a set of molecular probes with defined sizes to determine pore dimensions, and is also referred as chromatographic porosimetry [2].

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