Generally, methacrylic acid is used as a charge-bearing agent for generation of electroosmotic flow in capillary electrochromatography. However, methacrylic acid has a significant effect on the morphology of the monolithic stationary phases based on styrene – divinylbenzene system as showed recently by group in Prague. The monolithic material prepared without methacrylic acid in the polymerization [...]
Couple of days ago, I mentioned on Chromatographer’s facebook page article by Peter Carr about speed in HPLC published in Analytical Chemistry. There are two parts in the article: (i) critical comparison of different approaches how to reach speed in HPLC and (ii) theoretical background of speed and efficiency optimization in high performance liquid chromatography. [...]
Today, I would like to describe my favorite chromatographic books: from one I bought even before I (really) knew what chromatography is to one which has chapter with my name on it. Úvod do vysokoúčinné kapalinové kolonové chromatografie I am sorry to all of you who does not understand Czech language. This is my very [...]
Recently, I was browsing amazon.com products related to the chromatography keyword. To my big surprise, the number ten (at least in my results) was a “16 oz. Double Wall Insulated Tumbler with chromatography column alone – Paper Insert”. This is what I call a present for a chromatographer! I waited until our next order (Harry [...]
One of the most important characteristics describing the column properties is column permeability. Term permeability refers to the column packed with a stationary phase (particles or monolith) and describes how easy flows the liquid (mobile phase) through the column. At given pressure, the higher is flow through the column the higher is the permeability of [...]
UPDATED – One of the general property of the liquids is their resistance to change a form. This resistance is called viscosity and can be expressed as a resistance to flow. In case of liquids, the viscosity can be simply expressed as “thickness”. For example, water is “thin” with low viscosity, while honey is “thick” [...]
In liquid chromatography liquid mobile phase flows through the column with stationary phase. The main principle of separation remains the same. Compounds have different affinity to the stationary phase and are separated while flowing through the column. The compounds separated with liquid chromatography are disolved in the mobile phase. They have lower difussion coefficients than [...]
Thin layer chromatography is probably the easiest way how to perform chromatographic separation. At least you do not need any instrument. In thin layer chromatography (TLC) the solvent flows through the stationary phase which covers the thin plate. One part of plate is submerged into the mobile phase which travel across the plate using …
The gas chromatography is special type of chromatography, where the mobile phase is gas, such as helium or nitrogen. The stationary phase is usually solid support covered with liquid layer. After the sample injection the mobile phase carries the sample compounds through the column. Usually, the temperature gradient is applied and compounds are then separated [...]
Analytical chemist. Amateur photographer. Occasional traveler. Lazy graphoman. Wannabe entertainer. Boring moralist ...
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Chromatographic articles