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Separation of small molecules: surface area vs. gel porosity

Separation of small molecules: surface area vs. gel porosity

Generally, methacrylic acid is used as a charge-bearing agent for generation of electroosmotic flow in capillary electrochromatography. However, methacrylic acid has a significant effect on the morphology of the monolithic stationary phases based on styrene – divinylbenzene system as showed recently by group in Prague. The monolithic material prepared without methacrylic acid in the polymerization [...]

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How to get speed in HPLC

How to get speed in HPLC

Couple of days ago, I mentioned on Chromatographer’s facebook page article by Peter Carr about speed in HPLC published in Analytical Chemistry. There are two parts in the article: (i) critical comparison of different approaches how to reach speed in HPLC and (ii) theoretical background of speed and efficiency optimization in high performance liquid chromatography.

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My HPLC books

My HPLC books

Today, I would like to describe my favorite chromatographic books: from one I bought even before I (really) knew what chromatography is to one which has chapter with my name on it. Úvod do vysokoúčinné kapalinové kolonové chromatografie I am sorry to all of you who does not understand Czech language. This is my very [...]

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Chromatographic coffee cup – do it yourself

Chromatographic coffee cup – do it yourself

Recently, I was browsing amazon.com products related to the chromatography keyword. To my big surprise, the number ten (at least in my results) was a “16 oz. Double Wall Insulated Tumbler with chromatography column alone – Paper Insert”. This is what I call a present for a chromatographer! I waited until our next order (Harry [...]

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Column permeability

Column permeability

One of the most important characteristics describing the column properties is column permeability. Term permeability refers to the column packed with a stationary phase (particles or monolith) and describes how easy flows the liquid (mobile phase) through the column.

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