Theory

My HPLC books

by Jiri Urban on October 18, 2010 · 6 comments

My very first HPLC book

Today, I would like to describe my favorite chromatographic books: from one I bought even before I (really) knew what chromatography is to one which has chapter with my name on it. Úvod do vysokoúčinné kapalinové kolonové chromatografie I am sorry to all of you who does not understand Czech language. This is my very [...]

Liquid chromatography

by Jiri Urban on August 13, 2009 · 0 comments

Basic scheme of liquid chromatography instrument

In liquid chromatography liquid mobile phase flows through the column with stationary phase. The main principle of separation remains the same. Compounds have different affinity to the stationary phase and are separated while flowing through the column. The compounds separated with liquid chromatography are disolved in the mobile phase. They have lower difussion coefficients than [...]

Gas chromatography

by Jiri Urban on August 9, 2009 · 0 comments

Schematic GC instrument

The gas chromatography is special type of chromatography, where the mobile phase is gas, such as helium or nitrogen. The stationary phase is usually solid support covered with liquid layer. After the sample injection the mobile phase carries the sample compounds through the column. Usually, the temperature gradient is applied and compounds are then separated [...]

What is chromatography?

by Jiri Urban on August 7, 2009 · 0 comments

Scheme of chromatography

Chromatography is analytical chemistry method which is used (and useful) for the separation of complex mixtures of chemical compounds. The main mechanism of the separation is repeatable distribution of the tested compound in between two different phases. Usually, one phase is solid, fixed in the separation device and the other is moving and flows through [...]