
As I mentioned several weeks ago, there was an international symposium on the separation science – HPLC 2010 – held in Boston last week. It was my second North American conference (together with San Francisco 2006) and third in total (plus Stockholm 2005). Allow me to summarize my remarks I made during the lectures I [...]

Liquid chromatography offers numerous possibilities how to separate samples of interest. By changing the composition of mobile phase and/or character of stationary phase the separation efficiency and selectivity can be completely change. Read more about main liquid chromatography modes. Size-exclusion chromatography (SEC) Size-exclusion chromatography (also known as gel permeation chromatography, GPC) is separation technique, where [...]

In liquid chromatography liquid mobile phase flows through the column with stationary phase. The main principle of separation remains the same. Compounds have different affinity to the stationary phase and are separated while flowing through the column. The compounds separated with liquid chromatography are disolved in the mobile phase. They have lower difussion coefficients than [...]