From the category archives:

Theory

Chromatographic coffee cup – do it yourself

July 26, 2010

Recently, I was browsing amazon.com products related to the chromatography keyword. To my big surprise, the number ten (at least in my results) was a “16 oz. Double Wall Insulated Tumbler with chromatography column alone – Paper Insert”.
This is what I call a present for a chromatographer!
I waited until our next order (Harry Potter Playstation [...]

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Column permeability

February 16, 2010

One of the most important characteristics describing the column properties is column permeability. Term permeability refers to the column packed with a stationary phase (particles or monolith) and describes how easy flows the liquid (mobile phase) through the column.

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Mobile phase viscosity

January 15, 2010

UPDATED – One of the general property of the liquids is their resistance to change a form. This resistance is called viscosity and can be expressed as a resistance to flow. In case of liquids, the viscosity can be simply expressed as “thickness”. For example, water is “thin” with low viscosity, while honey is “thick” [...]

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Liquid chromatography

August 13, 2009

In liquid chromatography liquid mobile phase flows through the column with stationary phase. The main principle of separation remains the same.

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Thin layer chromatography

August 12, 2009

Thin layer chromatography is probably the easiest way how to perform chromatographic separation. At least you do not need any instrument. In thin layer chromatography (TLC) the solvent flows through the stationary phase which covers the thin plate. One part of plate is submerged into the mobile phase which travel across the plate using [...]

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Gas chromatography

August 9, 2009

The gas chromatography is special type of chromatography, where the mobile phase is gas, such as helium or nitrogen. The stationary phase is usually solid support covered with liquid layer.

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