Because of lack of small pores it is difficult to separate small molecules with polymer monoliths in isocratic mode. We have prepared monolithic capillary columns and then hypercrosslinked them to afford a monolith containing an array of small pores [1]. This monolithic column affords good separation of uracil and alkylbenzenes in isocratic mobile phase mode [...]
Yesterday, I had a great opportunity to participate in Discussion group organized by the An International Separation Science Society (CASSS). The main topic was the Scientific Achievements Award for one of the founders of modern HPLC – Dr. Jack Kirkland. Jack Kirkland is the inventor of superficially porous particle stationary phases. These particles have solid [...]
Superficially porous particles with thicker outer shells were used extensively for liquid-liquid chromatography [1] and as the support for early bonded-phase packings in reverse phase HPLC [2]. Structure of particles Nowadays superficially porous particles typically have a 5-µm solid core and a ~ 0.25 – 1 µm thick outer shell with 30-nm pores. But, the thinner the shell, the [...]
It is known that the kinetics of mass transfer in wide pore bonded silica can be slow, because of restricted intraparticle diffusion and, furthermore, remaining active surface sites can give rise to undesired interactions. All together, these effects cause additional peak dispersion in high performance liquid chromatography and often considerable loss in recovery of biological [...]
Analytical chemist. Amateur photographer. Occasional traveler. Lazy graphoman. Wannabe entertainer. Boring moralist ...
 |
 |
 |
 |
Chromatographic articles